Bioengineered muscle is emerging as a feasible regenerative medicine technology. This has been investigated most extensively in small sized pre-clinical models, with potential translation into humans being limited by constraints relating to scalability, cell alignment and tissue geometry. We aim to combinine existing knowledge of cell behaviour and traditional quantitative engineering approaches with new manufacturing technologies, notably 3D printing, to build human-scale constructs from a combination of living and synthetic materials that emulate behaviour of native muscle tissue. The project will generate new knowledge for translation into applications for regenerative medicine, drug screening, and in vitro tissue engineering models.
Nawroth et al. A tissue-engineered jellyfish with biomimetic propulsion. Nature Biotechnology 2012; 30:792-797 (doi:10.1038/nbt.2269)
Muses S, Morgan JE, Wells DJ (2011) A Novel Conditionally Immortal Muscle Cell-line for Investigating Therapeutic Strategies in Muscle Cell Research. PLoS ONE 6(9): e24826.
107. Godfrey C, Muses S, McClorey G, Wells KE, Coursindel T, Terry RL, Betts C, Hammond S, O’Donovan L, Hildyard J, El Andaloussi S, Gait MJ, Wood MJ, Wells DJ. (2015) How much dystrophin is enough: the physiological consequences of different levels of dystrophin in the mdx mouse. Hum Mol Genet. 24(15):4225-37..
Parmar, N., Day, R.M. (2015) TIPS to manipulate myogenesis: retention of myoblast differentiation capacity using microsphere culture. Eur Cell Mater. 30:41-50. doi:10.1155/2014/713631
Pedde et al. (2017) Emerging Biofabrication Strategies for Engineering Complex Tissue Constructs. Adv Mater. 29(19). doi: 10.1002/adma.201606061.