Development of novel optical imaging strategies to investigate metastasis at the molecular level

Simon Poland (primary)
Comprehensive Cancer Centre
King's College London
Simon Ameer-Beg (secondary)
King's College London


Whilst most of our understanding of the basic molecular principles of cancer have come from experimentation with single layer 2D cell culture models, they are not representative of the way cells interact in an in vivo environment. 3D cell cultures provide a much more realistic environment to simulate biological interactions and study invasiveness. Building on previous research this proposal will seek to develop novel optical imaging strategies which will enable for the first time, the complete interrogation of tumour derived 3D models, to understand how cancer cells are able to dissociate from the primary tumour and invade surrounding tissues.


S. P. Poland, G. K. Chan, J. A. Levitt, N. Krstajic, A. T. Erdogan, R. K. Henderson, M. Parsons, S. M. Ameer-Beg. Multifocal multiphoton volumetric imaging approach for highspeed time-resolved Förster resonance energy transfer imaging in vivo. Optics Letters, 43, 24, 6057-6060. 2018
N. Fili, Y. Hari-Gupta, Á. dos Santos, A. Cook, S. Poland, S. M Ameer-Beg, M. Parsons, C. Toseland. NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription. Nature Comms., 8, 1, 1871. 2017
C. A. Mitchell, S. P. Poland, J. Seyforth, J. Nedbal, T. Gelot, T. Huq, G. Holst, R. D. Knight, S. M. Ameer-Beg. Functional in vivo imaging using fluorescence lifetime light-sheet microscopy. Optics Letters, 42, 7, 1269-72. 2017
S. P. Poland, A. T. Erdogan, N..Krstajic, J. Levitt, V. Devauges, R. J, Walker, D.D.U., S.M. Ameer-Beg and R.K. Henderson. New high-speed centre of mass method incorporating background subtraction for accurate determination of fluorescence lifetime. Optics Express, 24, 7, 6899-6915. (2016).
N. Krstajic, S. Poland, J. Levitt, R. Walker, A. Erdogan, S. Ameer-Beg and R. K. Henderson 0.5 billion events per second time correlated single photon counting using CMOS SPAD arrays. Optics letters, 40, 18, 4305-4308 (2015).
S.P. Poland, N. Krstajic, J. Monypenny, S. Coelho, D. Tyndall, R. J. Walker, V. Devauges, J. Richardson, N. Dutton, P. Barber, D. D. U. Li, K. Suhling, Tony Ng,, R. K. Henderson, and S. M. AmeerBeg, “A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging,” Biomedical Optics Express 6.2, 277-296 (2015).
N. Krstajic, J. Levitt, S. Poland, S. Ameer-Beg and R. Henderson, “A 256 x 8 SPAD line sensor for time resolved fluorescence spectroscopy,” 23.5, 5653-5669 (2015).
S.P. Poland, N. Krstajic, R. D. Knight, R. K. Henderson, and S. M. Ameer-Beg, “Development of a doubly weighted Gerchberg-Saxton algorithm for use in multibeam imaging applications,” Optics Letters 39, 2431-2434 (2014).
S.P. Poland, N. Krstajic, S. Coelho, D. Tyndall, R. J. Walker, V. Devauges, P. E. Morton, N. S. Nicholas, J. Richardson, D. D. U. Li, K. Suhling, C. M. Wells, M. Parsons, R. K. Henderson, and S. M. Ameer-Beg, “Time-resolved multifocal multiphoton microscope for high speed FRET imaging in vivo,” Optics Letters 39, 6013-6016 (2014).
S.P. Poland, A. J. Wright, S. Cobb, J. C. Vijverberg, and J. M. Girkin, “A demonstration of the effectiveness of a single aberration correction per optical slice in beam scanned optically sectioning microscopes,” Micron 42, 318-323 (2011).
W. Lubeigt, S.P. Poland, G. J. Valentine, A. J. Wright, J. M. Girkin, and D. Burns, “Search based active optic systems for aberration correction in time-independent applications,” Applied Optics 49, 307-314 (2010).
S.P. Poland, A. J. Wright, and J. M. Girkin, “Evaluation of fitness parameters used in an iterative approach to aberration correction in optical sectioning microscopy,” Applied Optics 47, 731-736 (2008).
S.P. Poland, A. J. Wright, and J. M. Girkin, “Active focus locking in an optically sectioning microscope utilizing a deformable membrane mirror,” Optics Letters 33, 419-421 (2008).
J. Wright, S.P. Poland, J. M. Girkin, C. W. Freudiger, C. L. Evans, and X. S. Xie, “Adaptive optics for enhanced signal in CARS microscopy,” Optics Express 15, 18209- 18219 (2007).
Rousseau, S. Poland, J. M. Girkin, A. F. Hall, and C. J. Whitters, “Development of fibreoptic confocal microscopy for detection and diagnosis of dental caries,” Caries Research 41, 245-251 (2007).
J. Wright, D. Burns, B. A. Patterson, S.P. Poland, G. J. Valentine, and J. M. Girkin, “Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy,” Microscopy Research and Technique 67, 36-44 (2005).
J. M. Girkin, S. Poland, and C. Longbottom, Non-ionising imager, U.S. Patent Application 12/594,095. (2014).

Molecules, cells and industrial biotechnology
Area of Biology
Cell BiologyDevelopment
Techniques & Approaches
Image ProcessingMicroscopy / ElectrophysiologyMolecular Biology