Abstract
Stem cells are critical for regeneration, but this is impaired in ageing. Multiple cues regulate stem cell responses including metabolic and immune signalling. How these cues act to spatially regulate behaviour of stem cells during regeneration and how this affected by ageing is an urgent and important question. Zebrafish telomerase mutants will be used as to understand how ageing affects regeneration by characterising stem cell responses to injury and comparing to metabolite and lipid distribution. Combining live cell imaging, single cell sequencing, Raman imaging and DESI mass spectrometry imaging this project will identify novel ageing-related molecules regulating stem cell function.
References
Lleras-Forero L, Newham E, Teufel S, Kawakami K, Hartmann C, Hammond CL, Knight RD, Schulte-Merker S. Muscle defects due to perturbed somite segmentation contribute to late adult scoliosis. Aging (Albany NY). 2020 Sep 25;12(18):18603-18621. doi: 10.18632/aging.103856.
Høgset H, Horgan CC, Armstrong JPK, Bergholt MS, Torraca V, Chen Q, Keane TJ, Bugeon L, Dallman MJ, Mostowy S, Stevens MM. In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy. Nat Commun. 2020 Dec 2;11(1):6172. doi: 10.1038/s41467-020-19827-1.
Hwang AB, Brack AS. Muscle Stem Cells and Aging. Curr Top Dev Biol. 2018;126:299-322. doi: 10.1016/bs.ctdb.2017.08.008.
Knappe S, Zammit PS, Knight RD. A population of Pax7-expressing muscle progenitor cells show differential responses to muscle injury dependent on developmental stage and injury extent. Front Aging Neurosci. 2015 Aug 25;7:161. doi: 10.3389/fnagi.2015.00161.
Mitchell C, Caroff L, Solis-Lemus JA, Reyes-Aldasoro CC, Vigilante A, Warburton F, de Chaumont F, Dufour A, Dallongeville S, Olivo-Marin JC, Knight R. Cell Tracking Profiler – a user-driven analysis framework for evaluating 4D live-cell imaging data. J Cell Sci. 2020 Nov 23;133(22):jcs241422. doi: 10.1242/jcs.241422. PMID: 33093241; PMCID: PMC7710012.