Abstract
The interplay between the pancreatic epithelium and its surrounding microenvironment is pivotal for pancreas formation. This project will establish new methods for monitoring and manipulating the pancreatic matrix, then investigate the key microenviromental factors that drive pancreatic cell fate decisions. We will combine mathematical models with synthetic hydrogels, AFM-based stiffness mapping, and multiple particle tracking microrheology to characterize and modify the matrix around human induced pluripotent stem cell-derived pancreatic progenitor organoids. We will then use these tools to understand how changes in the matrix’s mechanical properties and diffusivity impacts pancreatic progenitors differentiation towards the beta cell fate.
References
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