All-optical interrogation of neural circuits in behaving animals

Michael Hausser (primary)
Wolfson Institute for Biomedical Research
University College London
Matteo Carandini (secondary)
UCL Institute of Ophthalmology


To understand how activity patterns in neural circuits drive behaviour, it is essential to not only read out activity patterns, but also to manipulate them on the spatial and temporal timescale that are relevant to the computations being carried out during behaviour. This project will harness a novel “all-optical closed loop” strategy for targeted manipulation of activity patterns based on real-time readout in order to enable ‘on-the-fly’ interventions in neural circuit dynamics during behaviour. By manipulating the right neurons at the right time we may be able to optimize network activity patterns to improve behavioural performance.


Robinson NTM, Descamps LAL, Russell LE, Buchholz MO, Bicknell BA, Antonov GK, Lau JYN, Nutbrown R, Schmidt-Hieber C, Häusser M (2020). Targeted Activation of Hippocampal Place Cells Drives Memory-Guided Spatial Behavior. Cell. 2020 Nov 6:S0092-8674(20)31302-7. doi: 10.1016/j.cell.2020.09.061.

Dalgleish HWP, Russell LE, Packer AM, Roth A, Gauld OM, Greenstreet F, Thompson EJ, Häusser M (2020). How many neurons are sufficient for perception of cortical activity? Elife. 2020 Oct 26;9:e58889.

Arlt C, Häusser M (2020). Microcircuit Rules Governing Impact of Single Interneurons on Purkinje Cell Output In Vivo. Cell Rep. 2020 Mar 3;30(9):3020-3035.e3.

Kostadinov D, Beau M, Blanco-Pozo M, Häusser M (2019). Predictive and reactive reward signals conveyed by climbing fiber inputs to cerebellar Purkinje cells. Nature Neurosci. 2019 Jun;22(6):950-962.

Zhang Z, Russell LE, Packer AM, Gauld OM, Häusser M (2018). Closed-loop all-optical interrogation of neural circuits in vivo. Nat Methods 5(12):1037-1040.

Animal disease, health and welfare
Area of Biology
Techniques & Approaches
Microscopy / Electrophysiology