The bacterial type II secretion system

Richard Pickersgill (primary)
SBCS
Queen Mary, University of London
Vidya Darbari (secondary)
SBCS
Queen Mary, University of London

Abstract

The type II secretion system delivers lytic enzymes and toxins into the environment causing crop spoilage and human and animal disease. We are exploring the structure of the type II secretion system using cryo-electron microscopy with the ultimate aim of elucidating how this molecular machine works.


References

  1. Rehman, S., Gu, S., Shevchik, V.E., Pickersgill, R.W. (2013) Anatomy of secretin-binding to the D. dadantii type II secretion system pilotin. Acta Crystallogr D Biol Crystallogr. 69, 1381-1386.
  2. Wang, X., Pineau, C., Gu, S., Guschinskaya, N., Pickersgill, R.W., Shevchik, V.E. (2012) Cysteine scanning mutagenesis and disulfide mapping analysis of the arrangement of GspC and GspD protomers within the T2SS. J. Biol. Chem. 287, 19082-19093.
  3. Gu, S., Kelly, G., Shevchik, V., Pickersgill, R.W. (2012) Solution structure of the HR domain and its interaction with the outer membrane secretin of the type II secretion system. J. Biol. Chem. 287, 9072-9080.
  4. Gu, S., Rehman, S., Shevchik, V.E., Pickersgill, R.W. (2012) Structural and functional insights into the pilotin-secretin complex of the type II secretion system. PLOS Pathogens 8: e1002531.
  5. Yang Y, Darbari VC, Zhang N et al. (2015) Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies. Science vol. 349, Article 6250, 882-885.

BBSRC Area
Plants, microbes, food and sustainability
Area of Biology
Structural Biology
Techniques & Approaches
BiochemistryBiophysicsMicroscopy / ElectrophysiologyMolecular Biology