Combined molecular and physiological mapping of olfactory representation in the brain

Yoh Isogai (primary)
46 Cleveland Street
University College London
Andreas Schaefer (secondary)
Neuroscience, Physiology & Pharmacology
University College London/ Francis Crick Institute


For many animals, olfaction is a critical sense. Animals acquire noxious and behaviorally important (food and social) information through scents, and smell often serves as a cue for specific memories. The logic of how smell, which typically consists of many odorants, forms a unique percept remains unclear. This project aims to map the molecular identities of the olfactory glomeruli, an elaborate structure that individual odorant inputs are relayed to the brain. In combination with in vivo calcium imaging of each glomerulus, we aim to create a map of the olfactory representation in the brain.


1 Robert Vassar, Steve K. Chao, Raquel Sitcheran, Jennifer M. Nunez, Leslie B. Vosshall, Richard Axel, Topographic organization of sensory projections to the olfactory bulb, Cell,Volume 79, Issue 6,1994, Pages 981-991
2 Kerry J. Ressler, Susan L. Sullivan, Linda B. Buck, Information coding in the olfactory system: Evidence for a stereotyped and highly organized epitope map in the olfactory bulb, Cell, Volume 79, Issue 7, 1994, Pages 1245-1255
3 Zapiec B, Mombaerts P. Multiplex assessment of the positions of odorant receptor-specific glomeruli in the mouse olfactory bulb by serial two-photon tomography. Proc Natl Acad Sci U S A. 2015;112(43):E5873–E5882. doi:10.1073/pnas.1512135112
4 Chen KH, Boettiger AN, Moffitt JR, Wang S, Zhuang X. RNA imaging. Spatially resolved, highly multiplexed RNA profiling in single cells. Science. 2015 Apr 24;348(6233):aaa6090. doi: 10.1126/science.aaa6090. Epub 2015 Apr 9. PubMed PMID: 25858977; PubMed Central PMCID: PMC4662681.
5 Wachowiak, M, Cohen, LB. Representation of Odorants by Receptor Neuron Input to the Mouse Olfactory Bulb, Neuron, Volume 32, Issue 4, 2001, Pages 723-735

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