Changes of cell shape are required for several cellular functions, including cell migration and mitosis. Cytoskeletal rearrangement, mechanical forces and changes in lipids and proteins local composition and/or activation are some of the processes responsible for cell shapes remodelling. In this project, we will combine biochemical, biophysical and computational studies to determine how a group of lipids collectively known as “phosphoinositides” (PIs) contributes to regulation of these processes. Specifically, we will determine the pattern of genetic alterations of PIs-regulating enzymes that occur in human cells and their impact on cytoskeletal remodelling, mechanical forces, PIs/proteins activation/localisation during migration/mitosis.
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