Combining bioengineering and cell biology to develop novel 3D models for studying bone remodelling in health and disease.

Dr Isabel Orriss (primary)
Comparative Biomedical Sciences
Royal Veterinary College
Dr Deepak Kalasker (secondary)
UCL Division of Surgery and Interventional Sciences
University College London


Recent advances in bioengineering and 3D printing have resulted in the development of 3D bioscaffolds for tissue repair and regeneration. Additionally, these scaffolds have the potential to become an essential tool in fundamental cell biology research. At present, most studies utilise cells grown in 2D on plastic, an approach that does not accurately mimic an in vivo situation. This studentship will use a combination of biological, bioengineering and biophysical techniques to design, optimise and validate a novel 3D bone cell culture model that will be used to improve our fundamental understanding of bone biology in health and disease.


Perpetuo IP, Bourne LE, Orriss IR (2019). Isolation and generation of osteoblasts. Methods Mol Biol 1914:21-38

Orriss IR, Arnett TR (2012). Rodent osteoclast cultures. Methods Mol Biol 816:103-117.

Cakmak, A. M., Unal, S., Sahin, A., Oktar, F. N., Sengor, M., Ekren, N., Kalaskar, D. M. (2020). 3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering. Polymers, 12 (9).

Aki, D., Ulag, S., Unal, S., Sengor, M., Ekren, N., Lin, C. -. C., kalaskar, D.M., Gunduz, O. (2020). 3D printing of PVA/hexagonal boron nitride/bacterial cellulose composite scaffolds for bone tissue engineering. Materials & Design, 109094.

Hexter, A. T., Shahbazi, S., Thangarajah, T., Kalaskar, D.M, Haddad, F. S., & Blunn, G. (2020). Characterisation of the tensile properties of Demineralised Cortical Bone when used as an anterior cruciate ligament allograft. Journal of the Mechanical Behavior of Biomedical Materials, 110.

Molecules, cells and industrial biotechnology
Area of Biology
BiotechnologyCell Biology
Techniques & Approaches
BiochemistryBiophysicsEngineeringImage ProcessingMicroscopy / ElectrophysiologyMolecular BiologySimulation / Modelling