Computerized analysis and modeling of temporal and spatial cell signaling

Paul Fromme (primary)
Mechanical Engineering
University College London
Stéphanie Kermorgant (secondary)
Bart Cancer Institute
Queen Mary, University of London

Abstract

Receptors transmit information from the cell surface but also from inside the cells, on endosomes. The aim is to understand how receptor tyrosine kinases (RTK) transmit information inside the cell and why intracellular RTK activity is required for optimal cell behavior. This project will apply engineering technology for the automatic imaging, quantification, and modeling of RTK “endosomal signaling” in human cells and tissues, combined with biochemistry and functional assays including cell migration, proliferation and survival. Novel technologies measuring and modeling receptor endosomal signaling will be generated, enhancing basic knowledge of receptor signaling and fitting with ENWW BBSRC enabling theme.


References

  1. Kermorgant S and Parker PJ. Receptor trafficking controls weak signal delivery: a strategy employed by c-Met for STAT3 nuclear accumulation J Cell Biol 18:855-63
  2. Joffre C, Barrow R, Ménard L, Calleja V, Hart IR and Kermorgant S. A direct role for Met endocytosis in tumorigenesis Nature Cell Biology 13:827-37
  3. Barrow-McGee R, Kermorgant S. Met Endosomal Signalling: In the right place, at the right time. Int J Biochem Cell Biol 2014, 49:69-74;
  4. Ménard L, Parker PJ, Kermorgant S. Receptor Tyrosine Kinase c-Met controls the cytoskeleton from different endosomes via different pathways. Nature Communications 2014, 5:3907
  5. Barrow-McGee, R. et al. Beta 1-integrin–c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes. Nature Communications 2016, 7:11942

BBSRC Area
Molecules, cells and industrial biotechnology
Area of Biology
AgeingCell Biology
Techniques & Approaches
BiochemistryBiophysicsEngineeringImage ProcessingMathematics / StatisticsMicroscopy / ElectrophysiologyMolecular BiologySimulation / Modelling