Abstract
Pathway analysis has become the first choice for gaining insight into the underlying biology of differentially expressed genes and proteins, as it reduces complexity and has increased explanatory power.
We aim to adopt the evolution of knowledge base–driven pathway analysis to elucidate aspects of the mitochondrial protein of our interest TSPO.
The most recent years of research have indeed pointed to a pleiotropic role for TSPO function that could imply a direct regulation of gene expression and their dependent pathways.
By combing the use of available libraries and genome wide analysis of our experimental models we will investigate this implementing the advanced approaches in bio-informatics.
References
Gatliff J and Campanella M. TSPO: kaleidoscopic 18kDa amid biochemical pharmacology, control and targeting of mitochondria. Biochem J. 2016 Jan 15;473(2):107-2. doi: 10.1042/BJ20150899.
Gatliff, J. et al. The 18 kDa protein TSPO interacts with VDAC1 and limits mitochondrial quality control. Autophagy 2014. Autophagy 2014 Dec 2;10(12):2279-96. doi:10.4161/15548627.2014.991665.
Sena LA and Chandel NS. Physiological roles of mitochondrial reactive oxygen species. Mol Cell. 2012 Oct 26;48(2):158-67. doi: 10.1016/j.molcel.2012.09.025.
Ristow M. Unraveling the truth about antioxidants: mitohormesis explains ROS-induced health benefits. Nat Med. 2014 Jul;20(7):709-11. doi: 10.1038/nm.3624
Wang J, et al. MitProNet: A knowledgebase and analysis platform of proteome, interactome and diseases for mammalian mitochondria. PLoS One. 2014 Oct 27;9(10):e111187.