MicroRNA-mediated silencing in mammalians regulates almost all fundamental cellular and biological processes. Central to this are four Argonaute (AGO) proteins, which are thought to have overlapping functions. However, our data and this exciting project will address how their redundancy is controlled by the association of LIMD1 family proteins (LIMD1, Ajuba and WTIP (LAW)). AGOs are part of the miRNA induced silencing complexes (miRISC), which also contains TNRC6 family proteins. The specific combinations of AGO/LAW/TNRC6 and the conditions/cell contexts (‘miRISC code’) that give rise to correct control of gene expression will be characterized in this novel study.
James et al., 2010 PNAS 107 (28) 12499-504
Bridge et al., 2017 Cell Reports 20 173-187
Schmid-Burgk et al., 2016 Nature Communications 7 12338
Gu et al., 2018 Genomics Proteomics Bioinformatics 16 136-143
Beltran et al., 2016 Genome Research 26 896-907