We will use genetically programmed protein ligation to assemble specific protein modules from molluscs/bacterial biofilms into novel adhesive fibres and gels. This will be achieved by combining our exclusive/innovative recombinant fusion protein system and our knowledge on bacterial biofilm/mollusc adhesion proteins. Utilising the recombinant fusion protein system, we will be able to self-assemble protein modules into the desired bio-adhesive. By combining fusions correctly, predictable arrays of adhesive fibres and gels will be formed with controllable properties/architectures. This technology offers a low-cost highly tuneable platform technology to produce novel adhesives.
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2. Harvey, J., Itzhaki, L.S. & Main E.R.G. (2018) “Programmed Protein Self-Assembly Driven by Genetically Encoded Intein-Mediated Native Chemical Ligation.” ACS Synth Biol. 7 (4), 1067-1074.
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