CRISPR and cruzi: Deciphering the ICL DNA REPAIRtoire of Trypanosoma cruzi using genome editingCRISPR, cruzi and the DNA REPAIRtoire: Deciphering the ICL repair systems of Trypanosoma cruzi using genome editing

Dr Shane Wilkinson (primary)
School of Biological & Chemical Sciences
Queen Mary University of London
Prof John Kelly (secondary)
Department of Pathogen Molecular Biology
London School of Hygiene and Tropical Medicine

Abstract

Interstrand crosslinks (ICLs) represent a lethal form of DNA damage, a property exploited by anticancer chemotherapies. To fix such lesions cells co-opte enzymes from different classical DNA repair pathways that together form an organism’s ‘ICL REPAIRtoire’. Using biochemical, cell biology and CRISPR-based functional genomic approaches, we plan to unravel how Trypanosoma cruzi, the parasite responsible for Chagas disease, repairs ICLs and investigate the interaction and interplay of the components that constitute this pathogen’s ‘ICL REPAIRtoire’. This will facilitate comparative studies against the ICL repair systems from other organisms, with any differences potentially exploitable in developing new drugs targeting Chagas disease.


References

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BBSRC Area
Molecules, cells and industrial biotechnology
Area of Biology
Cell BiologyMicrobiology
Techniques & Approaches
BiochemistryBioinformaticsMicroscopy / ElectrophysiologyMolecular Biology