Metazoan tissue phenotypes are emergent processes that supervene upon intercellular signalling between cells and intracellular signalling within cells. To study these processes, cell-type-specific signalling analysis of biomimetic tissue models is required. This project will develop a novel high-throughput air liquid interface (ALI) organoid platform that enables multiplexed cell-type-specific intracellular signalling (via mass cytometry) and intercellular signalling (via scRNA-Seq) analysis of cell-cell communication in tissues. We will use this technology to study how epithelial, stromal, and autologous immune cells communicate in the human colon.
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