Abstract
The project intends to develop and extend our recently developed super-resolution approach Super-Resolution Radial Fluctuation (SRRF) combining it with Light-Sheet Microscopy, namely diSPIM and Lattice Light-Sheet. By doing so, we will create a new 3D isotropic super-resolution method of unprecedented compatibility with long term live-cell imaging. We’ll then apply this system to study fundamental biology questions, such as the basis of T-cell signalling upon cell-cell interactions in 3D tissue scaffolds. The proposal combines the expertise in cell biology, optical physics and computational biophysics from two of the leading super-resolution research groups in the UK.
References
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