Abstract
In this project we plan to develop new analytical methods for in cell crosslinking mass spectrometry and also develop new computational methods to process, but also model protein complexes using the XL-MS data. We plan to use our new methods to identify the macromolecular machines mediating Fast Endophilin-Mediated Endocytosis (FEME) vesicle formation in vivo, a pathway that we have recently identified.
References
- Pandurangan AP, Vasishtan D, Alber F, Topf M. γ-TEMPy: simultaneous fitting of components in 3D-EM maps of their assembly using a genetic algorithm. (2015) Structure 23:2365–2376.
- Bullock JMA, Schwab J, Thalassinos K, Topf M. The importance of non-accessible crosslinks and solvent accessible surface distance in modelling proteins with restraints from crosslinking mass spectrometry. (2016) Mol Cell Proteomics. 15:10.1074/mcp.M116.05
- Boucrot E et al. (2015) ‘Endophilin marks and controls a clathrin-independent endocytic pathway’ Nature 517(7535):460-5
- Thalassinos K et al. (2013) ‘Conformational states of macromolecular assemblies explored by integrative structure calculation’ Structure, 21(9), pp1500-8