Exploring the dynamics of macromolecular assemblies using cryo EM and crosslinking mass-spectrometry

Maya Topf (primary)
Biological Sciences
Birkbeck College
Konstantinos Thalassinos (secondary)
Structural and Molecular biology


Determining structures of macromolecular assemblies is key to the mechanistic understanding of the cell. Due to the complexity of this task, it is often performed by a combination of multiple biophysical techniques complemented by computational methods. With recent advances in both cryo electron microscopy (cryo EM) and mass spectrometry techniques, a new dimension of assembly structures can be explored – their dynamics. We propose to develop a computational method for determining the architecture and dynamics of assemblies using integrative modelling based on information from cryo EM and cross-linking mass spectrometry. The method will be tested on known data and applied to assemblies from herpesviruses fusion machinery.


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Molecules, cells and industrial biotechnology
Area of Biology
EvolutionStructural Biology
Techniques & Approaches
BioinformaticsBiophysicsImage ProcessingMathematics / StatisticsSimulation / Modelling