Finding the dynamic structure of kinetochores using deep learning reconstruction

Susan Cox (primary)
Randall Centre
King's College London
Philip Auckland (secondary)
Randall Centre
King's College London


Mitosis is essential for the growth, development and homeostasis of eukaryotes. Kinetochores are protein machines that control mitotic progression by forming dynamic force-generating attachments to spindle microtubules. Here, we will use super-resolution microscopy to image large numbers of kinetochores at distinct mitotic stages at a resolution of tens of nm. A cutting-edge deep learning approach will be used to fit the data, where the training process of the network is used to optimise a model with no prior constraints being applied. This model will be integrated from data from live-cell experiments to create the first nanoscale model of kinetochore dynamics.


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Molecules, cells and industrial biotechnology
Area of Biology
Cell BiologyStructural Biology
Techniques & Approaches
BiochemistryBiophysicsImage ProcessingMicroscopy / ElectrophysiologyMolecular Biology