From manufacturing to in vivo efficacy: Establishing an interdisciplinary pipeline for new tools for gene delivery

Duncan Craig (primary)
School of Pharmacy
Stephanie Schorge (secondary)
Neuroscience, Physiology and Pharmacology


Gene delivery is a rapid growth area for a range of therapeutic applications; however progress is being held back by manufacturing considerations and this in turn is a reflection of the need for engineering, delivery, genetic and clinical knowledge to be incorporated into a single programme. Here we will use our interdisciplinary consortium to develop a project whereby nanogels are used to deliver genetic material to the brain as a novel method of non-viral delivery for the treatment of CNS disorders. We will provide a seamless programme that incorporates continuous manufacture via microfluidics, formulation and capacity optimisation, cellular transfection and clinical efficacy using in vivo models.


Whitely et al., 2021 “Microfluidic synthesis of protein-loaded nanogels in a coaxial flow reactor using a design of experiments approach” Nanoscale Advances

Snowball et al., 2019 “Epilepsy Gene Therapy Using an Engineered Potassium Channel” Journal of Neuroscience

Massaro et al., 2018 “Fetal gene therapy for neurodegenerative disease of infants” Nature Medicine
https://doi. org/10.1038/s41591-018-0106-7

Valitov et al., 2020 “Effect of acoustic streaming on continuous flow sonocrystallization in millifluidic channels” Chemical Engineering Journal

Tawfik et al., 2021 “In Vitro and In Vivo Biological Assessment of Dual Drug-Loaded Coaxial Nanofibers for the Treatment of Corneal Abrasion” International Journal of Pharmaceutics

Genes, development and STEM* approaches to biology
Area of Biology
Techniques & Approaches
BiochemistryBiophysicsChemistryEngineeringImage ProcessingMathematics / StatisticsMicroscopy / ElectrophysiologyMolecular Biology