Spectrins are conserved in all eukaryotes and they are thought to crosslink actin to the plasma membrane, forming a mechanically deformable skeleton that lies directly below the membrane. Although spectrins have been studied in erythrocytes, little is known about their function in other cell types. This project aims to characterise the function of the spectrin skeleton in cell migration in vivo, both in vertebrates and invertebrates. This will be achieved by analysing the function of spectrins in the migration of the primordial germ cells in fish and flies, as well as the migration of muscle stem cells in fish.
1 Garcia-Gonzalez. J., Brock A.J, Parker MO, Kuan V, Jolliffe D, Sudwarts A, Teh MT, Martineau AR, Walton RT and Brennan CH.(2020) Identification of Slit3 as a locus affecting nicotine preference in zebrafish and human smoking behaviour. eLife. Doi:10.7754/eLife.51295
2 Raimondi, C., Veronique Calleja, Riccardo Ferro, Alessandro Fantin, Andrew M. Riley, Barry V. L. Potter, Caroline H. Brennan, Tania Maffucci, Banafshé Larijani,& and Marco Falasca (2016) A Small Molecule Inhibitor of PDK1/PLCγ1 Interaction Blocks Breast and Melanoma Cancer Cell Invasion. Sci Rep. 20;6:26142. doi: 10.1038/srep26142
3 Drechsler M, Giavazzi F, Cerbino R and Palacios IM. (2017). Active diffusion and advection in the Drosophila ooplasm are due to the interplay of the microtubule and actin cytoskeletons. Nature Communications, doi: 10.1038/s41467-017-01414-6.
4 Ng BF, Selvaraj GK, Santa-Cruz Mateos C, Grosheva I, Alvarez-Garcia I, Martín-Bermudo MD and Palacios IM (2016). Alpha-Spectrin and Integrins act together to regulate actomyosin and columnarization, and to maintain a mono-layered follicular epithelium. Development. Apr 15;143(8): 1388
5 Knappe, S., Zammit, P., Knight, R. D. ‘A population of Pax7-expressing muscle progenitor cells show differential responses to muscle injury dependent on developmental stage and injury extent’. Frontiers in Aging Neuroscience (2015) 161. doi: 10.3389/fnagi.2015.00161.