Abstract
Phosphorylation is an integral part of cellular response to damaged DNA. This project aims to identify novel interactions between DNA damage response proteins dependent on phosphorylation, elucidate their function and detect new kinases which regulate them. We have developed and performed a large proteomic screen for protein interactions within DNA damage response activated or abolished by phosphorylation and likely carried out by kinases previously not identified as DNA damage response regulators. The student will take part in data analysis and validation of the screen and in functional characterization of 1-2 identified interactions, including identification of kinases responsible for their regulation.
References
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- Wilkes EH, Terfve C, Gribben JG, Saez-Rodriguez J, Cutillas PR. Empirical inference of circuitry and plasticity in a kinase signaling network. Proc Natl Acad Sci U S A. 2015;112(25):7719-7724.