Abstract
Autotrophic metabolism in bacteria and archaea is driven by electrochemical ion gradients across the plasma membrane to fix CO2 as organic molecules. Computational modelling and experimental work suggest that a rudimentary form of membrane heredity could have driven growth of protocells under alkaline hydrothermal conditions. This project will develop the initial computational modelling to consider the introduction of an energy currency (e.g. acetyl phosphate) in this setting, specifically analysing the prebiotic synthesis of amino acids, sugars and nucleotides. The project will test the model’s predictions in the lab, using microfluidic chips coupled to FTIR, Raman, GC-MS, LC-MS and HPLC.
References
1. Whicher A, Camprubi E, Pinna S, Herschy B, Lane N. Acetyl phosphate as a primordial energy currency at the origin of life.
Orig. Life Evol. Biosph. https://doi.org/10.1007/s11084-018-9555-8; 2018
2. West T, Sojo V, Pomiankowski A, Lane N. The origin of heredity in protocells. Phil. Trans. R. Soc. B. 372: 20160419; 2017
3. Martin W, Sousa FL, Lane N. Energy at life’s origin. Science 344: 1092-93; 2014
4. Sojo V, Pomiankowski A, Lane N. A bioenergetic basis for membrane divergence in archaea and bacteria. PLOS Biology 12:
e1001926; 2014.
5. Lane N, Martin WF. The origin of membrane bioenergetics. Cell 151: 1406-12; 2012.