Centromeres are the unique loci on eukaryotic chromosomes that ensure accurate chromosome segregation during cell division, by facilitating the assembly of large multi-protein complexes known as kinetochores. Centromeres and kinetochores are conserved throughout eukaryotes. The >100 proteins and protein complexes that constitute them are also largely conserved. However, the mechanisms by which each of these proteins and protein sub-complexes contribute to chromosome segregation are not well understood. This project will use cutting edge state-of-the-art genome-wide, proteome-wide and high-resolution imaging techniques to dissect how the CCAN network of proteins contribute to chromosome segregation in evolutionarily distant fission yeast and human cells.
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