Calcium is integral to cardiomyocyte contraction. Alterations to calcium uptake and handling are present in many cardiomyopathies. Hence, a method that can quantify calcium within live cells, animals and patients would give valuable information on cardiac physiology and pathology.
Manganese is a Calcium analogue which enters contractile cardiomyocytes through Calcium channels. Mn2+ levels can be quantified using magnetic resonance imaging-(MRI) and positron emission tomography-(PET) allowing interrogating cardiac function in living systems.
This project will develop new Mn2+-based imaging approaches and test their sensitivity to changes in Ca2+-handling within cultured cells and live mouse hearts using state-of-the-art in-vitro analysis and in-vivo imaging.
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Manganese-Enhanced Magnetic Resonance Imaging Enables In Vivo Confirmation of Peri-Infarct Restoration Following Stem Cell Therapy in a Porcine Ischemia-Reperfusion Model. (2015) Dash R, Kim PJ, Matsuura Y, Ikeno F, Metzler S, Huang NF, Lyons JK, Nguyen PK, Ge X, Foo CW, McConnell MV, Wu JC, Yeung AC, Harnish P, Yang PC. J Am Heart Assoc. Jul 27;4(7).
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PET Imaging of Cardiac Hypoxia: Hitting Hypoxia Where It Hurts. Victoria R. Pell, Friedrich Baark, Filipa Mota, James E. Clark, and Richard Southworth Cardiovasc Imaging Rep. 2018; 11(3): 7.