Receptor tyrosine kinases (RTK), playing major role in physiology and cancer, transmit information not only from the cell surface but also from inside the cells, on endosomes. Endosomes influence the intensity and the duration of the signaling. The aim is to generate methods to assess the RTK signaling spatially. This project will apply engineering technology for the automatic imaging, quantification, and modeling of RTK “endosomal signaling” in human cancer cells’ spheroids and tissues, combined to biochemistry and invasion-growth’s assays. Novel technologies measuring and modeling receptor endosomal signaling will be generated, enhancing knowledge of receptor signaling and influence on cell behavior.
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