Immunophysics is the application of biophysical tools to better understand the molecular mechanisms of immunological processes. In this project we will develop a novel advanced light microscopy system that combines optical tweezers and fast fluorescence imaging to study the trafficking and binding affinities of CTLA-4, a protein that is crucial in (negative) regulation of the immune response. The quantitative data that will be obtained by this project will enable a comprehensive understanding of CTLA-4 trafficking, internalization, recovery and recycling that is crucial for future therapies with CTLA-4 targeting drugs.
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