Inhibition of the soluble Epoxide Hydrolase by diet-derived lipid electrophiles: a combined biochemical and live-cell FTIR study.

Ka Lung Andrew Chan (primary)
Institute of Pharmaceutical Science
King's College London
Maria (Sasi) Conte (secondary)
Randall Division of Cell and Molecular Biophysics
King's College London

Abstract

Soluble epoxide hydrolase (sEH) is a key enzyme that modulates physiological and pathological cardiovascular functions through controlling levels of biologically active epoxide mediators (EETs).

sEH can be inhibited covalently by lipid electrophiles, the fatty acid nitroderivatives that can be endogenously synthesised in the gastrointestinal tract from dietary nitrates and unsaturated fatty acids (UFA), typical of a Mediterranean-style diet. However, molecular mechanisms are unclear. Here we aim to ally enzymatic and biophysical experiments in vitro with a novel live-cells FTIR model to characterise the molecular mechanisms and the functional effect of sEH inhibition by diet-derived nitro-FAs, in vitro and in cells.


References

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BBSRC Area
Animal disease, health and welfare
Area of Biology
Cell BiologyChemical Biology
Techniques & Approaches
BiochemistryBiophysicsChemistryMathematics / StatisticsMolecular Biology