Abstract
Determining structures of macromolecular assemblies is key to the mechanistic understanding of the cell. Due to the complexity of this task, it is often performed by a combination of multiple experimental techniques complemented by computational methods. We propose to develop a computational method for determining the architecture of dynamic assemblies at different levels of organisation using integrative modelling based on information from various mass spectrometry-based proteomic techniques. The method will be integrated into our general approach for assembly structure modelling (using primarily 3D-electron microscopy data) and will be tested on a number of protein assemblies, using both simulated and experimental data.
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