Label-free assessment of replicative senescence in flexor tendon fibroblasts

Jay Dudhia (primary)
Clinical Sciences and Services
Royal Veterinary College
ROger K Smith (secondary)
Clinical Sciences and Services
Royal Veterinary College


Cell senescence due to overuse (simulated in vitro by repeated cyclical loading) and ageing is a proposed mechanism for the failure of tissue remodelling leading to tendon degeneration and injury. Strategies that prevent senescence may be useful for treating this condition. The gold-standard for assessing senescence is immune-staining for senescence-associated β-galactosidase (SA-b-Gal) often involving invasive methodology that can modify cellular functions under investigation. The main aims are to investigate the (i) applicability of Raman spectroscopy (RS) as a label-free (non-invasive) approach to determine senescence in tendon tissues and isolated tendon fibroblasts. (ii) cellular pathways that lead to senescence in tendon fibroblasts.


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Animal disease, health and welfare
Area of Biology
AgeingCell Biology
Techniques & Approaches
BiochemistryBioinformaticsBiophysicsMathematics / StatisticsMicroscopy / Electrophysiology