Abstract
Cell senescence due to overuse (repeated cyclical loading) and ageing is a proposed mechanism for the failure of tissue remodelling leading to tendon degeneration and injury. Strategies that prevent senescence may be useful for treating this condition. The gold-standard for senescence is immune-staining for senescence-associated β-galactosidase (SA-b-Gal) often involving invasive preparation that can modify cellular functions under investigation. The main aims are to investigate (i) the cellular pathways that lead to senescence in tendon fibroblasts (ii) assess the applicability of Raman spectroscopy (RS) as a label-free approach to determine senescence in tendon tissues and isolated tendon fibroblasts.
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