How an organism responds to tissue loss after wounding is of considerable clinical interest. Slow or defective wound closure increases the risks of infection and can lead to ulceration, while over-healing can lead to scarring and even tumours. The aim of this project is to quantitatively understand how cytoskeletal machineries organize in space and time to regulate tissue repair. This will be achieved by developing an in silico model of the actin cytoskeleton, integrating molecular biochemistry, structure and mechanics. The model will be used to interpret and direct mechanical experiments on the Drosophila wing disc, combining genetics, live imaging and automated image analysis. Together, these approaches will determine how actomyosin structures dynamically reorganize to control tissue-scale mechanics during wound healing.
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