Abstract
Understanding the molecular processes involved in the formation and stabilization of neuronal networks requires efficient in vitro platforms to stimulate and interrogate developing brain-like structures. To this end, we will generate self-organised 3D cortical organoids (MiniBrains) that will be dynamically stimulated at precise locations with a NanoBionic interface. The proteomics and transcriptomics analysis of the MiniBrains throughout their development will reveal the molecular expression patterns. Dynamic imaging of fluorescent calcium reporter MiniBrains generated with CRISPR/Cas9 will correlate molecular expression and neuronal network activity patterns. This project combines nanotechnology, omics and organoids to investigate neuronal network development.
References
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