Multimodal Comparative Study of Matched Human iPSC and Biopsy-Derived Muscle Progenitor Cells for Regenerative Therapies

Charles Knowles (primary)
Blizard Institute, Barts and the London SMD
Queen Mary University of London
Richard Day (secondary)
UCL Division of Medicine
University College London


Skeletal muscle is an abundant tissue in the human body that can be functionally compromised due to trauma or a range of genetic, metabolic and neuromuscular diseases. Functional human skeletal muscle progenitor cells (myoblasts) can be derived from human induced pluripotent stem cells (hiPSC). This project will establish a high-content cell phenotyping platform combining morphological, cell marker staining and activation of cell signaling pathways to compare the phenotype of hiPSC-derived myoblasts with myoblasts isolated from donor skeletal muscle. The project will generate valuable new information regarding use of hiPSC-derived myoblasts for disease modelling, testing new therapeutics and developing regenerative medicines.


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(2) Wiseman et al. Integrated Multiparametric High-Content Profiling of Endothelial Cells. SLAS Discov. 2019;24:264-273. doi: 10.1177/2472555218820848.

(3) Leha et al. A high-content platform to characterise human induced pluripotent stem cell lines. Methods. 2016;96:85-96. doi: 10.1016/j.ymeth.2015.11.012.

(4) Parmar, N., Day, R.M. (2015) TIPS to manipulate myogenesis: retention of myoblast differentiation capacity using microsphere culture. Eur Cell Mater. 30:41-50. doi:10.1155/2014/713631

Molecules, cells and industrial biotechnology
Area of Biology
BiotechnologyCell Biology
Techniques & Approaches
BiochemistryBioinformaticsBiophysicsChemistryEngineeringImage ProcessingMathematics / StatisticsMicroscopy / ElectrophysiologyMolecular BiologySimulation / Modelling