Muscling in on skeletal muscle ischemia-reperfusion injury: modelling effects on the muscle spindle.

Darren Player (primary)
Division of Surgery and Interventional Science
Shervanthi Homer-Vanniasinkam (secondary)
Mechanical Engineering


Skeletal muscle ischemia-reperfusion injury (IRI) can manifest following a number of clinical scenarios, including; trauma, vascular injury and/or occlusion, haemorrhage and other haematological complications. It is now accepted that IRI is a consequence of acute inflammation, activated and controlled through mechanisms which are currently not fully elucidated. However, much of the focus to-date has been on damage to extrafusal tissue, with little attention being paid to effects on the muscle spindle. This project will aim to develop the first fully characterised humanised in vitro model of muscle spindle IRI that will provide a necessary platform for studies of the basic driving and consequential mechanisms, act a pre-clinical screening platform for therapeutic development, and importantly provide a robust translational animal replacement biotechnology.


Capel A;Rimington R;Fleming J; Player D ;Baker L;Turner M;Jones J;Martin N;Ferguson R;Mudera V et al. Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle
(10.3389/fbioe.2019.00020) Journal article : Frontiers in Bioengineering and Biotechnology 7(FEB):13 pages

Barrett, P, Quick, TJ, Mudera, V, Player, DJ, Generating intrafusal skeletal muscle fibres in vitro: Current state of the art and future challenges. Journal of Tissue Engineering, 2020. 11.

Martin NRW, Aguilar-Agon K, Robinson GP, Player DJ, Turner MC, Myers SD, Lewis MP. (2017), Hypoxia Impairs Muscle Function and Reduces Myotube Size in Tissue Engineered Skeletal Muscle. J Cell Biochem. 2017 Mar 10.

Homer-Vanniasinkam S, Rowlands TE, Hardy SC, Gough MJ. Skeletal muscle ischaemia-reperfusion injury: further characterisation of a rodent model. Eur J Vasc Endovasc Surg 2001; 22:523-527.

Homer-Vanniasinkam S, Granger D.N. Physiology of reperfusion injury. In White RA, Hollier LH (eds). Vascular Surgery: Basic Science and Clinical Correlations. Blackwell Publishing 2005; 245-250.

Genes, development and STEM* approaches to biology
Area of Biology
BiotechnologyCell BiologyPhysiology
Techniques & Approaches
BiochemistryEngineeringMicroscopy / ElectrophysiologyMolecular Biology