Skeletal muscle ischemia-reperfusion injury (IRI) can manifest following a number of clinical scenarios, including; trauma, vascular injury and/or occlusion, haemorrhage and other haematological complications. It is now accepted that IRI is a consequence of acute inflammation, activated and controlled through mechanisms which are currently not fully elucidated. However, much of the focus to-date has been on damage to extrafusal tissue, with little attention being paid to effects on the muscle spindle. This project will aim to develop the first fully characterised humanised in vitro model of muscle spindle IRI that will provide a necessary platform for studies of the basic driving and consequential mechanisms, act a pre-clinical screening platform for therapeutic development, and importantly provide a robust translational animal replacement biotechnology.
Capel A;Rimington R;Fleming J; Player D ;Baker L;Turner M;Jones J;Martin N;Ferguson R;Mudera V et al. Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle
(10.3389/fbioe.2019.00020) Journal article : Frontiers in Bioengineering and Biotechnology 7(FEB):13 pages
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