Navigating the Maze: Biotherapeutics Delivery Across the Epithelial Basement Membranes

Dr Driton Vllasaliu (primary)
Institute of Pharmaceutical Science
King's College London
Andela Saric (secondary)
Institute for the Physics of Living Systems


Achieving oral delivery of biotherapeutics is crucial for development of treatments accessible to everyone. Design of oral therapies requires understanding of how they cross the gastrointestinal tissue barrier. Multiple individual components of gastrointestinal mucosa (mucus, degradative enzymes, epithelium) contribute to the overall barrier, but the subepithelial basement membrane (BM) remains the least characterised barrier. Here we will characterize how biotherapeutics navigate the dense mesh of epithelial BM barrier. We will use state-of-the-art computer modelling and cell culture tools to quantify the diffusion of macromolecules across the intestinal epithelial BM, with a goal to inform on rational drug delivery and toxicology.


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Molecules, cells and industrial biotechnology
Area of Biology
PhysiologyStructural Biology
Techniques & Approaches
BiophysicsImage ProcessingMathematics / StatisticsMicroscopy / ElectrophysiologyMolecular BiologySimulation / Modelling