Abstract
Currently, microscopy is employed to study metabolic pathways and monitor the localisation and traffic of labelled proteins or metabolites. However, there are limitations with this approach because labels are unable to report any changes that might happen to a given metabolite. Our approach is to synthesise and validate a metabolite-based tracer technology to monitor ‘live’ metabolic changes in a signalling cascade that is important as a novel pharmacodynamic response marker to onco-therapeutics that perturb the PI3K-mTOR pathways. This will enable researchers to monitor localisation and turnover within a metabolic pathway simultaneously.
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