Abstract
Colistin is an antibiotic used to treat enteritis in livestock but is a last resort drug to treat gram negative resistant bacteria in people. There is an urgent need to re-assess the optimum dosage to preserve efficacy and limit the contribution of veterinary treatment to resistance across species. The project integrates:
– (i) the time-course of intestinal colistin concentration in chicken treated with clinical or subclinical dose (mass spectrometry measurement from in vivo samples),
– (ii) the effect on pathogens (static time-kill curves and ex vivo enumeration and susceptibility testing of E. coli)
– (iii) the effect on commensal gut flora (metagenomic 16S profiling)
References
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