Abstract
The nuclear compartment is highly dynamic and its integrity is constantly challenged by mechanical forces. Transient nuclear envelope ruptures during interphase (NERDI) occur when mechanical stress is generated by cytoskeletal forces, and NERDI repair requires membrane remodelling activities to reseal the nucleus. However, for repair to be effective, mechanical strain imposed by the cytoskeleton needs to be relieved from the nucleus to counteract intranuclear pressure. Here we will combine cell biology, advanced microscopy and Atomic Force Microscopy/Magnetic Tweezers to reveal the molecular mechanisms that coordinate membrane remodelling with mechanical forces regulation to facilitate nuclear envelope repair and maintain genome stability.
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