Uniquely amongst eukaryotes, Trypanosoma brucei RNA polymerase-I (RNAPI) transcribes a subset of mRNAs, including that for variant surface glycoprotein (VSG), the key mediator of antigenic variation. Research on T. brucei RNAPI transcriptional regulation has focused on its role in monoallelic VSG transcription, and relatively little is known about rDNA array transcriptional regulation. The T. brucei haploid genome contains nine rDNA arrays, only a subset of which are transcriptionally active. Using reporter cell lines, RNAi library selection and follow-up molecular analyses we will characterise the regulatory network of cis-acting elements and trans-acting factors that ensures optimal rRNA (and VSG mRNA) transcription.
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