Understanding how antimicrobials act on their target is key for developing new knowledge-based strategies to potentiate antimicrobial efficacy and critical for tackling antimicrobial resistance. Ribosomes are essential for protein synthesis and a major target for antimicrobials. Recently, a bacterial RNA repair system was found to maintain ribosome homeostasis by stabilising the RNA of the small ribosomal subunit and to increase tolerance to ribosome-targeting antimicrobials, representing a previously unrecognised physiological response. The student will now use a combination of molecular biology, biochemistry and single molecule biophysics techniques to mechanistically understand the impact of this system on ribosome function and antimicrobial tolerance.
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