Abstract
Gene activity is induced by extracellular stimuli via regulation of sequence specific transcription factors (TFs). Although we know much about the networks of signalling proteins that transmit information from the cell surface to TFs, is not clear how these signals alter the DNA binding properties of TFs in living cells. Here we will use single molecule imaging to monitor the DNA residence times of a TF that is crucial for developmental progression in response to extracellular signalling. We will then test how altering the magnitude and dynamics of extracellular signalling affects the residence time and binding frequency of the TF.
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