Abstract
Protein function is governed by the 3D structural fold, with health depending on the success of folding, and disease on its failure. Solving the question of how newly synthesised proteins fold to their functional states is considered as cracking the second half of the genetic code. However, most folding investigations target isolated, full length proteins, which is unrepresentative of cellular biosynthesis where proteins fold co-translationally as the ribosome is translating mRNA. This projects aims to probe co-translational folding of the understudied class of integral membrane proteins using high resolution NMR methods to elucidate the structure of the nascent protein.
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