Abstract
Neurotransmitter release from presynaptic vesicles at the synapse allows neurons to communicate in the brain. However the detailed spatial arrangement of different functional types of synaptic vesicles within the synapse remains unknown. We will use state-of-the-art super-resolution microscopy, including multi-colour, live and 3D STORM/STED microscopy, together with developing new analytical approaches, to address this question. We will then use these approaches to image changes occurring during synapse development, in response to neuronal activity and when key synaptic proteins are perturbed.
References
1 Andreae, L. C. & Burrone, J. Spontaneous Neurotransmitter Release Shapes Dendritic Arbors via Long-Range Activation of NMDA Receptors. Cell Rep, doi:10.1016/j.celrep.2015.01.032 (2015).
2 Andreae, L. C. & Burrone, J. The role of spontaneous neurotransmission in synapse and circuit development. J Neurosci Res 96, 354-359, doi:10.1002/jnr.24154 (2018).
3 Andreae, L. C., Fredj, N. B. & Burrone, J. Independent vesicle pools underlie different modes of release during neuronal development. The Journal of neuroscience : the official journal of the Society for Neuroscience 32, 1867-1874, doi:10.1523/JNEUROSCI.5181-11.2012 (2012).
4 Cox, S. et al. Bayesian localisation microscopy reveals nanoscale podosome dynamics. Nature Methods 9 195-200 2012
5 Cox, S. Super-resolution imaging in live cells. Dev Biol. 2015 May 1;401(1):175-81