Over 100 trillion synapses exist within a human nervous system. Local transcriptional changes orchestrate fundamental processes within each synapse. Recently, astrocytes emerged as a vital partner in establishing and maturing synapses, adding an additional layer of complexity, defined as the tripartite synapse. iPSC technology and advances in stem cell biology permit the generation of in vitro models of glial-neuronal interaction, and yet a functional model of astrocyte-neuronal interaction influencing the local synapse transcriptome is not available. This project takes advantage of bioengineering techniques, microfabrication and stem cell biology to generate the first human in vitro model of the tripartite synapse.
Hall CE, Yao Z, Choi M, Tyzack GE, Serio A, Luisier R, Harley J, Preza E, Arber C, Crisp SJ, Watson PMD, Kullmann DM, Abramov AY, Wray S, Burley R, Loh SHY, Martins LM, Stevens MM, Luscombe NM, Sibley C, Lakatos A, Ule J, Gandhi S, Patani R. ‘Progressive motor neuron pathology and the role of astrocytes in a human stem cell model of VCP-related ALS’. Cell Reports 2017 (Accepted)
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