Recent work demonstrates that receptors transmit information not only from the cell surface but also from inside the cells, on endosomes. The aim is to understand how receptor tyrosine kinases (RTK) transmit information inside the cell and why intracellular RTK activity is required for optimal cell behaviour. This project will apply engineering technology for the automatic imaging, quantification, and modelling of RTK “endosomal signalling” in human cancer cells and tissues, combined to biochemistry and functional assays including cell migration, proliferation and survival. Novel technologies measuring and modeling receptor endosomal signalling will be generated, enhancing basic knowledge of receptor signalling.
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