In adult tissues, stem cells maintain homeostasis by dividing to give rise to both new stem cells and differentiated cells. One mechanism by which stem cells balance self-renewal and differentiation is through competition between stem cells for space at the supportive micro-environment, or niche: cells which remain in the niche self-renew, while those pushed out differentiate. This project will use new genetic tools and computational tracking methods to visualise this process in an intact stem cell niche and understand how stem cells interact with each other and the niche to determine what fate to adopt.
1 Stem cells commit to differentiation following multiple induction events in the Drosophila testis.
Yuen, Hillion, Amoyel. bioRxiv 2021.05.20.444930; doi: https://doi.org/10.1101/2021.05.20.444930
2 Somatic stem cell differentiation is regulated by PI3K/Tor signaling in response to local cues. Amoyel, Hillion, Margolis, Bach. Development 2016 ;143(21):3914-3925. doi: 10.1242/dev.139782
3 Neutral competition of stem cells is skewed by proliferative changes downstream of Hh and Hpo.
Amoyel, Simons, Bach EMBO J. 2014 Oct 16;33(20):2295-313. doi: 10.15252/embj.201387500
4 Ulicna, Vallardi, Charras and Lowe (2020) Automated deep lineage tree analysis using a Bayesian single cell tracking approach. bioRxiv 2020.09.10.276980 doi: 10.1101/2020.09.10.276980
5 Bove, Gradeci, Fujita, Banerjee, Charras and Lowe (2017) Local cellular neighborhood controls proliferation in cell competition. Mol Biol Cell. 2017 Nov 7;28(23):3215-3228. doi: 10.1091/mbc.E17-06-0368