Shigella sonnei is an intracellular pathogen and the dominant cause of shigellosis globally. Zebrafish is a well-established model system to study host-pathogen interactions in vivo, and the Mostowy lab has recently shown it as valuable to study the pathogenesis and evolution of Shigella species. This project will use the Shigella-zebrafish infection model to investigate in vivo evolutionary phenomena indicated by recent genomics studies of S. sonnei from the Holt lab, including lineage-specific differences in host-pathogen interactions; functional effects of transposase activity (which interrupt some genes and up-regulate others); and in vivo fitness costs of AMR mechanisms in different strain backgrounds.
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